Anti-WNT7A Antibody Picoband™

Product Name

Anti-WNT7A Antibody Picoband™

View all Wnt-7a Antibodies

SKU/Catalog Number

A01728

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-WNT7A Antibody Picoband™ catalog # A01728. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-WNT7A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01728)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human WNT7A, identical to the related mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01728 is reactive to WNT7A in Human, Mouse, Rat

Applications

A01728 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

39-40 kDa

Calculated molecular weight

39.005kDa

Background of Wnt-7a

This gene is a member of the WNT gene family, which consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is involved in the development of the anterior-posterior axis in the female reproductive tract, and also plays a critical role in uterine smooth muscle pattering and maintenance of adult uterine function. Mutations in this gene are associated with Fuhrmann and Al-Awadi / Raas – Rothschild / Schinzel phocomelia syndromes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human, Mouse

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of WNT7A using anti-WNT7A antibody (A01728).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat liver tissue lysates,
Lane 4: mouse kidney tissue lysates,
Lane 5: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT7A antigen affinity purified polyclonal antibody (Catalog # A01728) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT7A at approximately 40 kDa. The expected band size for WNT7A is at 40 kDa.

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Figure 2. Flow Cytometry analysis of PC-3 cells using anti-WNT7A antibody (A01728).
Overlay histogram showing PC-3 cells stained with A01728 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WNT7A Antibody (A01728, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Click image to see more details

Figure 3. Flow Cytometry analysis of ANA-1 cells using anti-WNT7A antibody (A01728).
Overlay histogram showing ANA-1 cells stained with A01728 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WNT7A Antibody (A01728, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For WNT7A (Source: Uniprot.org, NCBI)

Alternative Names

protein Wnt-7a; proto-oncogene Wnt7a protein; wingless-type MMTV integration site family, member 7A; Wnt7a; Wnt-7a WNT7A Wnt-7a Wnt family member 7A protein Wnt-7a|proto-oncogene Wnt7a protein|wingless-type MMTV integration site family, member 7A

For more info on WNT7A, check out the WNT7A Infographic

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In this infographic, you will see the following information for WNT7A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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